Amyloid-Beta 1-40, E22G, 15N Uniform Label (0.5 mg) Human, Recombinant

Description

Amyloid-Beta 1-40, Glu22Gly, ¹⁵N Uniform Label (Artic variant)                                                                          

Amount

0.5mg

Format

Lyophilized

Sequence

DAEFRHDSGYEVHHQKLVFFAGDVGSNKGAIIGLMVGGVV

Purity

95% by Chromatography and SDS-PAGE

Counter Ion

Ammonium Acetate

Solubility

Alexotech has developed a proprietary technique of preparing highly soluble Amyloid β-peptides.  It is however, of utmost importance to follow our recommendations of solubilisation and to efficiently solubilise the Amyloid β-peptide the pH should briefly be raised to between 11-12. This can be accomplished by addition of e.g. 20 mM NaOH, however, at higher peptide concentrations a higher concentration of NaOH may be required due to the intrinsic buffering capacity of the peptide. The pH should therefore always be monitored and if necessary adjusted. After solubilisation the pH can be adjusted using a 10X stock solution of the buffer of choice

Storage

Store at -20°C upon arrival.

Source

Protein expressed in Escherichia coli in minimal media using ¹⁵NH₄Cl as sole nitrogen source.