Amyloid-Beta 1-40, 13C, 15N Uniform Label (0.5 mg) Human, Recombinant

Description

Recombinant Amyloid-Beta-Peptide (1-40) uniformly 13C,15N labeled

Amount

0.5mg

Format

Lyophilized

Sequence

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV

Purity

95% by Chromatography and SDS-PAGE

Counter Ion

Ammonium Acetate

Solubility

Alexotech has developed a proprietary technique of preparing the amyloid β-peptide (1-40) having a superior solubility. It is however, of utmost importance to follow our recommendations of solubilisation: To efficiently solubilise the amyloid β-peptide (1-40) the pH should briefly be raised to between 11-12. This can be accomplished by 20 mM of NaOH, however, at high peptide concentrations a higher NaOH concentration may be required due to the intrinsic buffering capacity of the peptide. The pH should therefore always be monitored and if necessary adjusted. After solubilisation the pH can be adjusted using the buffer of choice.

Storage

Store at -20°C upon arrival.

Source

Protein expressed in Escherichia coli using ¹⁵NH₄-Cl as sole nitrogen source and ¹³C glucose as sole carbon source.