SOD1 80-96, Human Superoxide Dismutase 1 Soluble, Mouse, Monoclonal

Immunogen

KLH-conjugated synthetic peptide derived from human SOD1 sequence P00441
Peptide used to elicit this antibody is not conserved in SOD2, 3 and 4.

Host

Mouse

Clonality

Monoclonal

Subclass/isotype

IgG1, (clone number 210.29)

Purity

Affinity purified

Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

ELISA (ELISA), Western Blot (WB)

Recommended Dilution

1:1000-1:10 000 (ELISA), 1:1000 (WB)

Expected | apparent MW

15.9 kDa

Confirmed Reactivity

Human

Predicted reactivity

Bovine, Chimpanzee, Dog, Goat, Guinea Pig, Mouse, Pig, Rabbit, Rat, Schizosaccharomyces pombe, Sheep

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Application examples

100,10 and 1 ng of recombinant human SOD1 were separated by 4-20 % SDS-PAGE and transferred electrophoretically (25V, 10 min) onto PVDF membrane. Non-specific binding sites were blocked by incubating membrane with 5 % dry milk in PBS, 0.1 % Tween 20 for 1h at room temperature (RT) with agitation. The membrane was thereafter incubated with the primary antibody SOD1 aa 58-72 at a dilution of 1: 1 000 for 3h at RT with agitation. The antibody solution was decanted and the membrane was rinsed 3 times for 5 min in PBS-T (0.05 %) at RT with agitation. The membrane was then incubated with the secondary antibody (Rabbit Anti-Mouse IgG – HRP conjugated (DAKO) at a 1:1 000 dilution) for 1h at RT with agitation. The membrane was washed as above and developed for 5 min with Amersham ECL prime western blotting detection reagent according to the manufacturer’s instructions (GE Healthcare). Exposure time was 15 s.

Related products

Available antibodies targeting Human SOD1

Background

SOD1 [Cu-Zn] (EC=1.15.1.1) is a cytoplasm localized oxidoreductase which destroys radicals normally produced within the cells and toxic to biological systems. Alternative names: SOD, soluble, indophenoloxidase A, Cu/Zn superoxide dismutase, superoxide dismutase, cytosolic.

Product citations

To be added when available.