Description
Article no.: AS08 328
Product Information
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Immunogen |
Synthetic peptide chosen from human Abeta (1-42) protein. Amino acid sequence: |
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Host |
Rabbit |
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Clonality |
Polyclonal |
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Purity |
Serum |
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Format |
Lyophilized |
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Quantity |
100 µl |
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Reconstitution |
For reconstitution add 100 µl of sterile water. |
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Storage |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
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Tested applications |
Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL), Western blot (WB) |
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Recommended Dilution |
1 : 1000 (DB), 1 : 3000 (ELISA), 1-2 µg/ml (IL) |
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Expected | apparent MW |
4.5 kDa |
Reactivity
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Confirmed Reactivity |
Human |
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Predicted Reactivity |
Bovine, Chicken, Dog, Porcine, Rabbit |
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Not reactive in |
No confirmed exceptions from predicted reactivity are currently known. |
Application Examples
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Application examples |
Dot blot and Western analysis
 *Abeta (1-42) consists of both monomeric and partly aggregated mtrl. |
Additional Information
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Additional information |
The antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17). This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting.Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit. |
Related Products
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Related products |
Background
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Background |
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. |
Product Citations
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Product citations |
Lindhagen-Persson et al. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928. |