Anti-Amyloid-Beta, OMAB, Mouse, Monoclonal

Immunogen

Partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42). The epitope is 3-8. Molecular weight of immunogen is 4.5 kDa. Oligomer specific.

Host

Mouse

Clonality

Monoclonal

Subclass/isotype

IgM

Purity

Affinity purified

Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 100 µl of sterile water.

Storage

Store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

ELISA (ELISA), Immunolocalization (IL)

Recommended Dilution

Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)

Expected | apparent MW

4.5 kDa

Confirmed Reactivity

Human Abeta oligomers only

Predicted reactivity

Rat

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Application example

Abeta oligomer-specific antibody was adsorbed to Nunc-Immuno MaxiSorp plates (Nunc, Roskilde, Denmark) at 2 ug/ml in PBS. 1 ml of a 10 uM Aβ(1-42) sample containing a small fraction of Aβ-oligomers was separated using a superdex G75 (10/30) column. Aβ-fractions collected from the SEC were allowed to bind to OMAB plates for 20 minutes at 0°C. All fractions were analyzed and bound Aβwas detected using a polyclonal rabbit anti-Aβ antibody (AS08 328), Agrisera AB, Vännäs, Sweden) at a 1:1000 dilution followed by an anti-rabbit HRP-conjugated secondary antibody at a 1:5000 dilution (GE healthcare). ECBlue (Medicago, Uppsala, Sweden) was used as a substrate for HRP and the signal was detected by measuring the absorbance at 450 nm. Blocking solution and antibody-dilutions were made with 5% Non-fat dry milk in PBST and all washes were performed with PBS containing 0.1% Tween-20 (PBST).

10 µm of coronal sections from fresh-frozen transgenic mouse brain mutant (A) and wild type (B). Post-fixation in 4% formaldehyde solution, 5 min. OMAB antibody diluted 1:500, incubation at 4ºC ON. Mouse on mouse HRP-Polymer kit according to company instructions. Biocare Medical: BC-MM510 (Histolab) DAB substrate kit for peroxidase. Vector Laboratories: SK-4100 (ImmunKemi) Counterstained with Mayers HTX.

Additional information

OMAB antibody is a versatile tool within research of Alzheimer’s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

Related products

Available antibodies for Alzheimer's disease

Background

Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to their labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Product citations

Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of Aβ Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401.

Kumar et al. (2017). Foldamer-Mediated Structural Rearrangement Attenuates Aβ Oligomerization and Cytotoxicity. J Am Chem Soc. 2017 Nov 29;139(47):17098-17108. doi: 10.1021/jacs.7b08259. Zhao et al. (2016).

 Antiamyloidogenic Activity of Aβ42-Binding Peptoid in Modulating Amyloid Oligomerization. Small. 2016 Oct 7. doi: 10.1002/smll.201602857.

Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.

Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.