Anti-Amyloid-Beta, mAB-M, Mouse, Monoclonal

Immunogen

Synthetic peptide chosen from human Amyloid-Beta protein (3-10) region, oligomer specific.

Host

Mouse

Clonality

Monoclonal

Subclass/isotype

IgG1, kappa light chain, (clone number 2D10.F6)

Purity

Affinity purified

Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

For short time storage please add sodium azide and srote at +4°C.
For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes

Tested applications

Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL)

Recommended Dilution

10 ug/ml (IL), 1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture)

Expected | apparent MW

4.5 kDa

Confirmed Reactivity

Human

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Application examples

Dot blot

Dot blot reaction of the binding capacity of mAB-M to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-mAB-M (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).

Immunolocation

IHC used to illustrate the lack of binding of mAB-M to plaques. Tissue sections from the human AD hippocampus were de-waxed and rehydrated in ethanol and then incubated with AS08 357 (A) and mAB-M(B) at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).

Additional information

Due to location of antigen used to elicit this antibody in 3-10 region, it should bind to full length APP

Related products

Available antibodies for Alzheimer's disease

Background

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.

Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently published technique a highly oligomer-specific antibody (mAB-M), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.

Product citations

Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.