Description
Article no.: AS08 358
Product Information
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Immunogen |
Recombinant, full length, human Alpha-Synuclein UniProt: P37840. |
|
Host |
Rabbit |
|
Clonality |
Polyclonal |
|
Purity |
Serum |
|
Format |
Lyophilized |
|
Quantity |
100 µl |
|
Reconstitution |
For reconstitution add 100 µl of sterile water. |
|
Storage |
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
|
Tested applications |
ELISA (ELISA), Immunohistochemistry (IHC), Western Blot (WB) |
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Recommended Dilution |
1 : 3000 (ELISA), 1-2 µg/ml (IL), 1 : 1000 (WB) |
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Expected | apparent MW |
14 kDa |
Reactivity
|
Confirmed Reactivity |
Human |
|
Predicted reactivity |
Primates, Chimpanzee, Gorilla |
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Not reactive in |
No confirmed exceptions from predicted reactivity are currently known. |
Application Examples
|
Application examples |
Western Blot
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Additional Information
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Additional information |
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit. |
Related Products
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Related products |
Background
|
Background |
Alpha-synuclein is normally an unstructured soluble protein that can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy-bodies, and multiple system atrophy. |
Product Citations
|
Product citations |
Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857. |