Anti-Alpha-Synuclein, ASyO2, Mouse, Monoclonal, Oligomer specific

Immunogen

Synthetic peptide derived from human Alpha-Synuclein, amino acids 131-140.

Host

Mouse

Clonality

Monoclonal

Subclass/isotype

IgG1, (clone number 51.24)

Purity

Affinity purified

Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

For short time storage add sodium azide and store at +4°C.
For long time storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC)

Recommended Dilution

1-2 ug/ml (Dot), 2-4 ug/ml (ELISA capture), 10 ug/ml (IHC)

Expected | apparent MW

14 kDa

Confirmed Reactivity

Human

Predicted reactivity

Mouse

Not reactive in

No confirmed exceptions from predicted reactivity are currently known.

Additional information

This antibody is specific for Human Alpha-Synuclein oligomer structure.

Application examples

Dot Blot

Dot blot reaction of the binding capacity of ASyO2 to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-ASyO2 (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).

Immunolocalization

Tissue sections from the human PD midbrain, substantia nigra, were de-waxed and rehydrated in ethanol and then incubated with ASyO2 at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).

Related products

Available antibodies targeting Alpha-Synuclein