Amyloid-Beta 1-42 (1.0 mg) Human, Synthetic

Description

Synthetic Amyloid-Beta-peptide 1-42

Amount

1mg

Format

Lyophilized

Molecular Weight

 4514 Da

Sequence

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

Purity

95% HPLC and SDS-PAGE

QC Data

Mass Spectrometry
- Observed mass: 4514.08 Da
- Theoretical mass: 4514.10 Da

Note: The images and data shown represent a typical QC batch and may not correspond to the current lot.

Counter Ion

TFA (Trifluoracetic Acid)

Solubility

Alexotech has developed a proprietary method for preparing the amyloid β-peptide with superior solubility. It is, however, crucial to follow our recommended solubilization procedure. To effectively dissolve the amyloid β-peptide, the pH should be briefly raised to between 11 and 12. This can be achieved by adding 20 mM NaOH. At higher peptide concentrations, a greater NaOH concentration may be required due to the peptide’s intrinsic buffering capacity. The pH should therefore always be monitored and adjusted as necessary. After solubilization, the pH can be brought to the desired level using the buffer of choice.

Storage

Store at -20°C upon arrival.

Product Citation

Lindhagen-Persson, M., Brännström, K., Vestling, M., Steinitz, M., & Olofsson, A. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PloS one, 5(11), e13928. doi:10.1371/journal.pone.0013928

Lindberg, D. J., Wranne, M. S., Gilbert Gatty, M., Westerlund, F., & Esbjörner, E. K. (2015). Steady-state and time-resolved Thioflavin-T fluorescence can report on morphological differences in amyloid fibrils formed by Aβ(1-40) and Aβ(1-42). Biochemical and Biophysical Research Communications, 458(2), 418–423. https://doi.org/10.1016/j.bbrc.2015.01.132 

Olofsson, A., Lindhagen-Persson, M., Vestling, M., Sauer-Eriksson, A. E., & Öhman, A. (2009). Quenched hydrogen/deuterium exchange NMR characterization of amyloid-β peptide aggregates formed in the presence of Cu2+or Zn2+. FEBS Journal, 276(15), 4051–4060. https://doi.org/10.1111/j.1742-4658.2009.07113.x

Brunetti, D., Torsvik, J., Dallabona, C., Teixeira, P., Sztromwasser, P., Fernandez-Vizarra, E., … Bindoff, L. A. (2015). Defective PITRM1 mitochondrial peptidase is associated with A  amyloidotic neurodegeneration. EMBO Molecular Medicine, 8(3), 176–190. https://doi.org/10.15252/emmm.201505894